Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (PFKFB2) ELISA Kit,Abbkine_specification/price/image

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6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate.This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene.

Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (PFKFB2) ELISA Kit employs a two-site sandwich ELISA to quantitate PFKFB2 in samples. An antibody specific for PFKFB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFKFB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKFB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKFB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (PFKFB2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/mouse-6-phosphofructo-2-kinase-fructose-26-biphosphatase-2-pfkfb2-elisa-kit-kte71453/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.