The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications .
Mouse Bifunctional protein NCOAT (MGEA5) ELISA Kit employs a two-site sandwich ELISA to quantitate MGEA5 in samples. An antibody specific for MGEA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMGEA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MGEA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MGEA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Bifunctional protein NCOAT (MGEA5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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