MIF4GD encodes a protein which interacts with the N-terminus of the stem-loop binding protein (SLBP) and the 3' end of histone mRNA. This interaction facilitates the activation of histone mRNA translation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Mouse MIF4G domain-containing protein (MIF4GD) ELISA Kit employs a two-site sandwich ELISA to quantitate MIF4GD in samples. An antibody specific for MIF4GD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMIF4GD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIF4GD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIF4GD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse MIF4G domain-containing protein (MIF4GD) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-mif4g-domain-containing-protein-mif4gd-elisa-kit-kte71024/
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