Myo15 encodes an unconventional myosin. The myosin XV differs from other myosins in that it has a long N-terminal extension preceding the conserved motor domain. Studies in mice suggest that this protein is necessary for actin organization in the hair cells of the cochlea. Mutations in this gene have been associated with profound, congenital, neurosensory, nonsyndromal deafness. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Read-through transcripts containing an upstream gene and this gene have been identified, but they are not thought to encode a fusion protein. Several alternatively spliced transcript variants have been described, but their full length sequences have not been determined.
Mouse Myosin-XV (MYO15A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO15A in samples. An antibody specific for MYO15A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO15A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO15A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO15A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Myosin-XV (MYO15A) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-myosin-xv-myo15a-elisa-kit-kte70879/
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