Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.Sequence analysis predicted that the 346-amino acid SFRP4 protein contains an N-terminal signal peptide, no transmembrane domain, and a hydrophilic C terminus. In situ hybridization analysis demonstrated exclusive expression in stromal and myometrial cells, particularly in endometrium and breast. Upregulated expression was detected in endometrial and breast carcinomas.
Mouse Secreted frizzled-related protein 4 (SFRP4) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP4 in samples. An antibody specific for SFRP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFRP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Secreted frizzled-related protein 4 (SFRP4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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