Mouse Sphingosine-1-phosphate lyase 1 (SGPL1) ELISA Kit,Abbkine_specification/price/image

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Sphingosine-1-phosphate (SPP) participates in the proliferative signal transduction pathways of mammalian cells. SPP synthesis is catalyzed by sphingosine kinase, and SPP degradation is catalyzed by SPP lyase (SPL). The first SPL gene cloned, BST1 ('bestowed of sphingosine tolerance'), was isolated from budding yeast . A yeast mutant strain containing a deletion of the entire BST1 coding region is sensitive to the growth-inhibitory effects of D-erythro-sphingosine, due to its inability to degrade SPP. Spl, that has 59% sequence similarity to the BST1 gene product. They showed that this cDNA can functionally complement the yeast BST1 deletion, restoring a sphingosine-resistant phenotype. Northern blot analysis detected Spl expression in several mouse tissues, with the highest level found in the liver.

Mouse Sphingosine-1-phosphate lyase 1 (SGPL1) ELISA Kit employs a two-site sandwich ELISA to quantitate SGPL1 in samples. An antibody specific for SGPL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySGPL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SGPL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SGPL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Sphingosine-1-phosphate lyase 1 (SGPL1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.