TMED8 contains 1 GOLD domain.Members of the p24 (p24/gp25L/emp24/Erp) family of proteins have been shown to be critical components of the coated vesicles that are involved in the transportation of cargo molecules from the endoplasmic reticulum to the Golgi complex. The p24 proteins form hetero-oligomeric complexes and are believed to function as receptors for specific secretory cargo.The identification of the GOLD domain could aid in directed investigation of the role of the p24 proteins in the secretion process. The newly detected group of GOLD-domain proteins, which might simultaneously bind membranes and other proteins, point to the existence of a novel class of adaptors that could have a role in the assembly of membrane-associated complexes or in regulating assembly of cargo into membranous vesicles.
Mouse Protein TMED8 (TMED8) ELISA Kit employs a two-site sandwich ELISA to quantitate TMED8 in samples. An antibody specific for TMED8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMED8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMED8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMED8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Protein TMED8 (TMED8) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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