Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification.The three substrates of this enzyme are xanthine, NAD+, and H2O, whereas its three products are urate, NADH, and H+.This enzyme belongs to the family of oxidoreductases, to be specific, those acting on CH or CH2 group with NAD+ or NADP+ as acceptor.Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.
Mouse Xanthine dehydrogenase/oxidase (XDH) ELISA Kit employs a two-site sandwich ELISA to quantitate XDH in samples. An antibody specific for XDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XDH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XDH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Xanthine dehydrogenase/oxidase (XDH) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-xanthine-dehydrogenase-oxidase-xdh-elisa-kit-kte70003/
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