The secreted protein encoded by CYR61 is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation.
Human Cysteine-rich 61 (Cyr61/CCN1) ELISA Kit employs a two-site sandwich ELISA to quantitate CYR61 in samples. An antibody specific for CYR61 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYR61 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYR61 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYR61 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Cysteine-rich 61 (Cyr61/CCN1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-cysteine-rich-61-cyr61-ccn1-elisa-kit-kte62153/
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