DACH2 is one of two genes which encode a protein similar to the Drosophila protein dachshund, a transcription factor involved in cell fate determination in the eye, limb and genital disc of the fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions. DACH2 is located on the X chromosome and is subject to inactivation by DNA methylation. The encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role in premature ovarian failure. Multiple transcript variants encoding different isoforms have been found for DACH2.
Human Dachshund homolog 2 (DACH2) ELISA Kit employs a two-site sandwich ELISA to quantitate DACH2 in samples. An antibody specific for DACH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDACH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DACH2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DACH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Dachshund homolog 2 (DACH2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-dachshund-homolog-2-dach2-elisa-kit-kte62151/
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