The E. coli heat-shock protein DnaJ is the founding member of a family of proteins that are associated with protein folding, complex assembly, and export. Prokaryotic and eukaryotic DnaJ homologs contain an approximately 70-amino acid J domain, which is thought to mediate interaction with 70-kD heat-shock proteins .The HSPF2 gene is located approximately 250 bp centromeric of the VEGFB gene. The HSPF2 coding sequence is contained in 5 exons. The deduced 241-amino acid HSPF2 protein has a J domain followed by a predicted transmembrane domain. The human and mouse HSPF2 proteins are 71% identical. Northern blot analysis detected an approximately 1.4-kb HSPF2 transcript in all human cell lines examined.
Human DnaJ homolog subfamily C member 4 (DNAJC4) ELISA Kit employs a two-site sandwich ELISA to quantitate DNAJC4 in samples. An antibody specific for DNAJC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNAJC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNAJC4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNAJC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human DnaJ homolog subfamily C member 4 (DNAJC4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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