Dynamin-1 is a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity.More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.
Human Dynamin-1 (DNM1) ELISA Kit employs a two-site sandwich ELISA to quantitate DNM1 in samples. An antibody specific for DNM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Dynamin-1 (DNM1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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