Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts.Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Four alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined.
Human Dynamin 2 (DNM2) ELISA Kit employs a two-site sandwich ELISA to quantitate DNM2 in samples. An antibody specific for DNM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Dynamin 2 (DNM2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-dynamin-2-dnm2-elisa-kit-kte61994/
bio-equip.cn
中文
