Human DNA (cytosine-5)-methyltransferase 1 (DNMT1) ELISA Kit,Abbkine_specification/price/image

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DNA (cytosine-5)-methyltransferases (DNMTs), such as DNMT1, maintain patterns of methylated cytosine residues in the mammalian genome. Genomic methylation patterns are reshaped during gametogenesis and early development and undergo programmed alterations during cellular differentiation. Methylation patterns are responsible for the repression of parasitic sequence elements and the expression status of genes subject to genomic imprinting and X inactivation. Faithful maintenance of methylation patterns is required for normal mammalian development, and aberrant methylation patterns are associated with certain human tumors and developmental abnormalities.A DNMT1 enzyme is thought to be responsible for most of the maintenance as well as the de novo methylation activities occurring in the somatic cells of vertebrates.

Human DNA (cytosine-5)-methyltransferase 1 (DNMT1) ELISA Kit employs a two-site sandwich ELISA to quantitate DNMT1 in samples. An antibody specific for DNMT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNMT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNMT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNMT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human DNA (cytosine-5)-methyltransferase 1 (DNMT1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-dna-cytosine-5-methyltransferase-1-dnmt1-elisa-kit-kte61993/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.