KLK5 belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. For background information on kallikreins.Using a positional candidate approach to identify additional KLK genes on 19q13.3-q13.4, followed by EST database searching and PCR, Yousef and Diamandis (1999) obtained a cDNA encoding KLK5, which they called KLKL2. RT-PCR analysis detected expression primarily in brain, mammary gland, and testis, with lower levels in other tissues.Kim et al. (2001) noted that KLK5 is expressed in a subset of ovarian tumors. They found a strong positive correlation between KLK5 expression and tumor grade and disease stage. They concluded that KLK5 expression is associated with more aggressive forms of epithelial ovarian carcinoma.
Human Kallikrein-5 (KLK5) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK5 in samples. An antibody specific for KLK5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Kallikrein-5 (KLK5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-kallikrein-5-klk5-elisa-kit-kte61896/
bio-equip.cn
中文
