Human Kininogen-1 (KNG1) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

High molecular weight kininogen (HMWK) plays an important role in assembly of the plasma kallikrein -kinin system. The KNG1 gene generates both HMWK and low molecular weight kininogen (LMWK) through alternative splicing. Both HMWK and LMWK contain an identical heavy chain consisting of protein domains 1, 2, and 3. However, HMWK contains a 56-kD light chain that consists of domains 5 and 6H, whereas LMWK contains a unique 4-kD light chain that consists of domain 5L. In both proteins, the heavy and light chains are linked by domain 4, which contains the bradykinin (BK) nonapeptide. BK, which is released by plasma kallikrein, is a potent inflammatory mediator that causes vasodilation and enhanced capillary permeability, induces pain, and stimulates production of nitric oxide and prostacyclin from endothelial cells.

Human Kininogen-1 (KNG1) ELISA Kit employs a two-site sandwich ELISA to quantitate KNG1 in samples. An antibody specific for KNG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKNG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KNG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KNG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Kininogen-1 (KNG1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-kininogen-1-kng1-elisa-kit-kte61890/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.