Human V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

Like other members of the Ras family, the KRAS protein is a GTPase and is an early player in many signal transduction pathways. KRAS is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. When mutated, KRAS is a oncogene. The protein product of the normal KRAS gene performs an essential function in normal tissue signaling, and the mutation of a KRAS gene is an essential step in the development of many cancers.KRAS acts as a molecular on/off switch, once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. KRAS binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the terminal phosphate of the nucleotide converting it to GDP.

Human V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) ELISA Kit employs a two-site sandwich ELISA to quantitate KRAS in samples. An antibody specific for KRAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRAS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRAS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-v-ki-ras2-kirsten-rat-sarcoma-viral-oncogene-homolog-kras-elisa-kit-kte61889/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.