Human Lysophosphatidylcholine acyltransferase 2 (LPCAT2) ELISA Kit,Abbkine_specification/price/image

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LPCAT2 encodes a member of the lysophospholipid acyltransferase family. The encoded enzyme may function in two ways: to catalyze the biosynthesis of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) from 1-O-alkyl-sn-glycero-3-phosphocholine, and to catalyze the synthesis of glycerophospholipid precursors from arachidonyl-CoA and lysophosphatidylcholine.The protein may function in membrane biogenesis and production of platelet-activating factor in inflammatory cells. The deduced full-length protein contains an N-terminal catalytic domain and 2 cation-binding EF-hand motifs in its C-terminal region.Epitope-tagged Lpcat2 was expressed mainly in the ER and Golgi of transfected Chinese hamster ovary cells.

Human Lysophosphatidylcholine acyltransferase 2 (LPCAT2) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT2 in samples. An antibody specific for LPCAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPCAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPCAT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPCAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Lysophosphatidylcholine acyltransferase 2 (LPCAT2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.