MDM1encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles, is amplified in the mouse transformed 3T3 cell line, and the encoded protein is able to bind to p53. Alternatively spliced transcript variants encoding different isoforms have been found for MDM1.
Human Nuclear protein MDM1 (MDM1) ELISA Kit employs a two-site sandwich ELISA to quantitate MDM1 in samples. An antibody specific for MDM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Nuclear protein MDM1 (MDM1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-nuclear-protein-mdm1-mdm1-elisa-kit-kte61677/
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