Human Mediator of RNA polymerase II transcription subunit 1 (MED1) ELISA Kit,Abbkine_specification/price/image

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The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. Mediator of RNA polymerase II transcription subunit 1 encoded by MED1 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. Mediator of RNA polymerase II transcription subunit 1 is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. Mediator of RNA polymerase II transcription subunit 1 is known to have the ability to self-oligomerize.

Human Mediator of RNA polymerase II transcription subunit 1 (MED1) ELISA Kit employs a two-site sandwich ELISA to quantitate MED1 in samples. An antibody specific for MED1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Mediator of RNA polymerase II transcription subunit 1 (MED1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.