Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit,Abbkine_specification/price/image

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The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by MED12, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in MED12 cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome.

Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12 in samples. An antibody specific for MED12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-mediator-of-rna-polymerase-ii-transcription-subunit-12-med12-elisa-kit-kte61671/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.