Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by MMP-14 is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
Human Matrix Metalloproteinase 14 (MMP-14) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP14 in samples. An antibody specific for MMP14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Matrix Metalloproteinase 14 (MMP-14) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-matrix-metalloproteinase-14-mmp-14-elisa-kit-kte61598/
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