1. General
Ochratoxin is a mycotoxin produced by the fungus Aspergillus ochraceous and also by several species of Penicillium fungi. Ochratoxin has been known to cause kidney damage and decreased egg production in chickens. It is an immunosuppressant and is considered a potential carcinogen.
Ochratoxin A immunoaffinity columns can be used with AOAC Official Methods for the measurement of ochratoxin in baby food,barley, beer, wine,green coffee and roasted coffee.
2. Intended Use
Ochra Test is quantitative method for the detection of ochratoxin A in a variety of commodities. Such as grain, feed, soybean, rapeseed, vegetable oil, soy sauce, vinegar, jam and liquor.These products are safe and simple. Sample clean up can be performed in less than 15 minutes and determined by HPLC or LC-MS. It is a fast, simple, safe and highly accurate method for quantitatively measuring Ochratoxin A.
3.Principle
To measure ochratoxin levels, samples are prepared by mixing with an extraction solution, followed by blending and filtering. The extract is then applied to the Ochratoxin A column, which contains specific antibodies for Ochratoxin A. At this stage, the ochratoxin binds to the antibody on the column. The column is then washed to rid the immunoaffinity column of impurities. By passing methanol through the column, the ochratoxin is removed from the antibody. The methanol can then be injected into an HPLC or LC-MS system.
Wuhan Ecalbio Co., Ltd
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Address: HZAU, Nanhu Road, Wuhan city 430075, China.
Tel: +86 27 87745856
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E-mail: info@ecalbio.com
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