Illumina, Agilent Technologies to Co-Market Targeted Resequencing Solution

New Offering Brings Together Long Reads on Illumina's Genome Analyzer with Agilent's SureSelect Platform to Substantially Simplify Workflow

Illumina Inc. (NASDAQ: ILMN) and Agilent Technologies (NYSE: A) today announced they have entered into a non-exclusive co-marketing agreement to support a new, scalable solution for researchers conducting targeted resequencing studies. The Agilent SureSelect Target Enrichment System, optimized for Illumina's widely adopted next-generation sequencing platform, the Genome Analyzer, provides researchers with a method for efficiently resequencing specific regions of interest in the genome. This cost-effective approach significantly simplifies the lab process while providing even coverage and high alignment rates. Ultimately, the Agilent SureSelect Target Enrichment System, when combined with Illumina's Genome Analyzer, allows researchers to conduct studies that otherwise would not be feasible.

Illumina and Agilent are highlighting the benefits of their combined solution at the American Association of Cancer Research (AACR) meeting this week in Denver, Colo. The new research protocol enables researchers to interrogate multiple regions of interest to detect rare mutations, such as those commonly found in cancer, while taking advantage of low sample input requirements. Targeted resequencing also enables researchers to sequence areas identified through genome-wide association studies in addition to sequencing candidate genes and candidate regions.

Chad Nusbaum, co-director of the Genome Sequencing and Analysis program at MIT's Broad Institute, described the hybrid selection technique utilized by Agilent's SureSelect technology in Nature Biotechnology, February 2009: "We have developed a hybrid-selection method for enriching specific subsets of a genome that is flexible, scalable and efficient. Targeting based on hybrid selection may be potentially useful for a variety of applications where traditional single-plex PCR is either too costly or too specific. Examples are enrichment of precious ancient DNA that is heavily contaminated with unwanted DNA, deep sequencing of viral populations in clinical samples, or metagenomics analyses of environmental or medical specimens."

"Our partnership with Agilent underscores our commitment to build upon the utility of our next-generation sequencing technology, helping researchers plan and execute studies at a scale never before possible," said Jay Flatley, president and CEO of Illumina. "We continue to expand our portfolio of solutions with powerful tools for studying genetic variation. This solution provides the research community with a cost-effective, automation-friendly and flexible approach to targeted resequencing for a wide variety of applications."

"The Agilent SureSelect Target Enrichment System requires only standard equipment already in the next-generation sequencing workflow," said Nick Roelofs, Ph.D., Agilent's vice president and general manager, Life Science Solutions Unit. "The SureSelect platform draws upon Agilent's distinctive ability to custom-manufacture oligos greater than 100 base pairs accurately and reliably, resulting in highly specific capture of genome variance. When combined with Illumina's Genome Analyzer, researchers can design their own experiments and take advantage of Illumina's long reads to achieve timely, reproducible results while reducing cost and increasing robustness." The SureSelect System enables researchers to easily and efficiently design custom content through eArray probe design and incorporate automation for scalability and cost efficiencies.

For more information, visit www.illumina.com/resequencing or www.opengenomics.com/SureSelect.

About the Illumina Genome Analyzer

Designed for facilities of all sizes, the Illumina Genome Analyzer has been adopted across genome centers worldwide, as well as individual research labs, core and service facilities, and biotechnology and pharmaceutical companies. The Genome Analyzer offers the highest rate of daily output and the simplest, most user-friendly workflow. The Genome Analyzer supports the broadest set of applications, including those used to profile and discover novel transcripts, to create high-resolution genome-wide maps of DNA-protein binding sites and to sequence entire human genomes to greater than 30x coverage. For more information about the Genome Analyzer and to read what customers are doing with Illumina's sequencing technology, please visit www.illumina.com/sequencing.

About the SureSelect Target Enrichment System

The SureSelect Target Enrichment System is a unique tool for greatly streamlining next generation sequencing research by enabling scientists to sequence only genomic areas of interest. When sequencing only a particular segment of the genome, the Agilent SureSelect platform can be used to capture a subset of exons or other genome targets and wash away the rest of the genome. SureSelect replaces other labor-intensive methods of targeted re-sequencing such as polymerase chain reaction (PCR) techniques that are a major bottleneck in most next-generation sequencing workflows.