Postnova Analytics : Efficient Purification of Extreme Viruses

Postnova Analytics announce in a joint collaboration with the Department of Biosciences at the University of Helsinki (Finland), that Asymmetrical Flow Field-Flow Fractionation (AF4) was successfully employed for the purification of halophilic (extreme) viruses.

Viruses come in a wide range of shapes and sizes, of particular current interest to researchers are viruses that live in extreme conditions such as high salinity or elevated temperature. One challenge for studying these extreme viruses has been to identify an efficient separation technique which both purifies and maintains their infectious nature.

 

In a recently published paper**, by the University of Helsinki and Postnova Analytics, the researchers report how they used a Postnova AF2000 system for purifying and studying extremophilic viruses in four morphotypes forms - lemon-shaped, tailed, tailless icosahedral and pleomorphic enveloped. 

Experimental data is shown illustrating how the Postnova AF2000 system was applied to input samples of different purity including crude supernatants of infected cultures, polyethylene glycol-precipitated viruses and viruses purified by ultracentrifugation. All four virus morphotypes were successfully purified by the system. AF4 purification of culture supernatants or polyethylene glycol-precipitated viruses yielded high recoveries, and the purities were comparable to those obtained by the multistep ultracentrifugation purification methods. In addition, the researchers also demonstrate how they have successfully employed the Postnova AF2000 system as a rapid monitoring tool for virus production in slowly growing host cells living in extreme conditions.

The Postnova AF2000 is a high performance Asymmetrical Flow Field-Flow Fractionation (AF4) system for separation of proteins, macromolecules and nanoparticles. The system uses a gentle crossflow chromatography-like technique for size-based separation that does not use a solid stationary phase and the mobile phase composition is readily adjustable according to the sample needs. Offering gentle, high resolution separation of specimens up to 50 µm, the AF4 technique is shown in the paper to be highly suitable for virus purification. 

For further information on AF2000 system please visit www.postnova.com/af2000-overview.html or contact Postnova Analytics on +44-1885-475007 / info.uk@postnova.com. To read the University of Helsinki / Postnova Analytics paper in full please visit http://www.postnova.com/news-message/items/new-publication-out-purification-of-halophilic-viruses.html 

Founded in 1997, Postnova Analytics is the inventor and leading international supplier of Field-Flow Fractionation (FFF) systems for markets including biopharmaceuticals, polymers, materials, nanotechnology and environmental sciences. Leveraging its unique and patented modular FFF - Light Scattering Platform, Postnova has been able to provide high performance solutions to a wide range of applications. Today the company's growing product portfolio includes Flow FFF, Centrifugal FFF, Thermal FFF, Size Exclusion Chromatography (SEC), MALS and DLS Light Scattering.  Postnova (www.postnova.com) is located and headquartered in Landsberg am Lech (Germany) and has subsidiaries in the USA, UK and Finland. These offices, in conjunction with a highly qualified and trained distribution network, provide informed local support to customers worldwide.

Worldwide HQ 

Postnova Analytics GmbH

Max-Planck-Straße 14
86899 Landsberg am Lech

Germany 

Tel:+49-8191-985-6880

Email: info@postnova.com

Web www.postnova.com

 

UK

 

Postnova Analytics UK Ltd.

Unit 56, The Hop Store

Bishops Frome

WR6 5BT

UK

Tel: +44-1885-475007

Email:info.uk@postnova.com

Web www.postnova.com

 

North America

 

Postnova Analytics Inc
230 South 500 East, S. #120
Salt Lake City, Utah 84102
USA

Tel:+1 801 521 2004

Email:info@postnova.com

Web www.postnova.com