IDT: New Application Note on Optimization of CRISPR-Cas9

New application note gives researchers insights and guidance on maximizing HDR-mediated insertion of short fragments in CRISPR genome editing

CORALVILLE, IA (November 16, 2017) – As an advocate for the genomics age, Integrated DNA Technologies (IDT), has published a new application note on optimization of CRISPR-Cas9 for homology-directed repair (HDR) applications. Available as part of the IDT collection of CRISPR application notes and protocols, this article supports researchers using CRISPR in their genome editing studies to maximize HDR rate in knock-in and other short-insertion experiments.

 

Described as ‘true genome editing’, HDR is the most precise and error-free repair process available for breaks introduced in CRISPR experiments. However, due to the generally low baseline rate of HDR, optimizing reagents and experimental conditions becomes critical to maximize HDR-mediated insertions. This application note offers practical advice from IDT genomics experts, based on thorough optimization studies. This includes experiment planning, sequence design, preparation of reagents, delivery of CRISPR components, and verification of inserts.

 

This new resource adds to IDT’s extensive and continually updated library of genomics topics and underlines IDT’s commitment to developing new tools and techniques for genome editing and supporting scientists with up-to-date educational content for success in their work.

 

For more information, please visit www.idtdna.com, or follow IDT on twitter @idtdna for real-time updates and insights.

 

About IDT

Integrated DNA Technologies, Inc. (IDT) develops, manufactures, and markets nucleic acid products for the life sciences industry in the areas of academic and commercial research, agriculture, medical diagnostics, and pharmaceutical development. IDT has developed proprietary technologies for genomics applications such as next generation sequencing, CRISPR genome editing, qPCR, and RNA interference. Through its GMP services, IDT manufactures products used in diagnostic tests for many forms of cancer and most inherited and infectious diseases. Serving over 100,000 life sciences researchers and producing over 65,000 nucleic acids daily, IDT is widely recognized as the industry leader in custom nucleic acid manufacture. IDT has its manufacturing headquarters in Coralville, Iowa, USA, with additional manufacturing sites in San Diego, California, USA; Leuven, Belgium; and Singapore.

 

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