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Porvair: Biological Sample Preparation Using a Protein Precipitation Plate

hits:661     Date:11/10/17
Porvair Sciences has published an informative video** demonstrating how to use a solid-state filter microplate to remove interfering proteins from serum, plasma or even whole blood samples prior to analysis by Liquid Chromatography / Mass Spectrometry (LC/MS).

Biological samples commonly contain proteins that interfere with downstream applications. The Porvair Sciences Protein Precipitation Plate (P3) plate uses the CRASH method, in which the protein is denatured with acetronitrile and the flocculant filtered out, allowing 96 samples to be handled at one time. 

Based on the industry standard MicroLute™ format, but without the chromatographic sorbent, protein 鈥榗rashes鈥 out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated with the CRASH technique of protein clean-up. The novel dual frit, hydrophobically treated matrix used by the P3 plate means that there is no 鈥榳etting out鈥 and leakage of sample before the application of vacuum. Porvair Sciences P3 plate is now commonly used as the protein precipitation plate of choice in many major pharmaceutical companies. 

To watch this video please visit https://www.youtube.com/watch?v=atPM7CaOFsU**.  For further information on the P3 plate please visit http://www.porvair-sciences.com/en/services-menu/solid-phase-extraction/p3-protein-precipitation-plates/ or contact Porvair Sciences on +44-1978-666222) or email  int.sales@porvair-sciences.com .

Established in 1992, Porvair Sciences is one of the largest global manufacturers of ultra-clean microplates for life science, synthetic chemistry and many other applications.  Porvair Sciences Ltd. and JG Finneran are wholly owned subsidiaries of Porvair plc.

Worldwide HQ

Porvair Sciences Ltd

Clywedog Rd South
Wrexham Industrial Estate
Wales LL13 9XS
Tel: +44 1978 666222  
Email: int.sales@porvair-sciences.com 
Web www.porvair-sciences.com
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