Abbkine Cell counting kit-8 is a colorimetric assay kit for cell number counting, cell prolifiration and cytotoxicity assays. By adding CCK-8 directly to the cell media, CCK-8 kit shows fast, high-throughput screening without a solubilization process, obtaining highly reproducible and accurate results.

Cell Counting Kit 8 (CCK8) uses the reagent WST-8 as its substrate. The highly water-soluble tetrazolium salt, WST-8 is cleaved to a soluble formazan by cellular dehydrogenases. This bioreduction is accomplished by NADPH or NADH produced by dehydrogenase enzymes in metabolically active cells. The amount of formazan dye product directly correlates to the number of metabolically active cells in the culture. 

The results obtained by using the cck8 show higher sensitivity than methods that use tetrazolium compounds such as MTT, MTS, XTT or WST-1, with lesser time and toxicity. 
1. More convenient with less than 15 mins in 3 steps
2. More sensitive than MTT, MTS, XTT or WST-1
3. Less toxic,  compatible for other downstream assays
4. High quality product with affordable price

HeLa Cell: MEM, 10% FCS, L-Glu 
Incubation: 37 °C, 5% CO2, 2  hours 
Detection: CCK-8 (450 nm), MTS (490 nm), MTT (570 nm)

HL60 Cell: RPMI 1640, 10% FCS, L-Glu  
Incubation: 37 °C, 5% CO2, 2  hours 
Detection: CCK-8 (450 nm), MTS (490 nm), MTT (570 nm)

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.