The recombinant human M-CSF protein, His Tag is expressed in Human Cells, amino acid sequence derived from N-terminal fragment (Met 1-Asn 190) of human CSF1 (P09603-1) was fused with a polyhistidine tag at the C-terminus. Human and Cynomolgus CSF1 sequences are identical.

The recombinant human CSF1 consists of 169 amino acids and predictes a molecular mass of 19.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCSF1 is approximately 27-32 kDa band due to glycosylation.

Human M-CSF protein, His Tag measured by its binding ability in a functional ELISA.
1. Immobilized human CSF1R (Cat:10161-H08H) at 10 μg/mL (100 μl/well) can bind biotinylated human CSF-1 (Cat:11792-H08H). The EC50 of biotinylated human CSF-1 (Cat:11792-H08H) is 8 ng/mL.
2. Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.4-2.3 ng/mL.

Abbkine suplies sizes: 5µg, 20µg, 100µg of Human M-CSF protein, His Tagfor your choice, more detailed information at https://www.abbkine.com/product/human-m-csf-protein-his-tag-prp100265.

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.