The recombinant human VEGF165 protein is expressed in Baculovirus-Insect Cells, whose amino acid sequence is derived from human VEGF 165 isoform (P15692-4), (Met1-Arg191) was expressed. Human and Cynomolgus VEGF165 sequences are identical.

The recombinant human VEGF consists of 165 amino acids after removal of the signal peptide and predicts a molecular mass of 19.2 kDa. Due to different glycosylation, the apparent molecular mass of rhVEGF165 is approximately 20 and 22 kDa in SDS-PAGE under reducing conditions, corresponding to the monomer.

Human VEGF165 protein measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 3-12 ng/ml .

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