The recombinant human EGF protein is expressed in E. coli, whose amino acid sequence is derived from mature form of human EGF (NP_001954.2), (Asn 971-Arg 1023) was expressed and purified, with an initial Met at the N-terminus.

The recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.3 kDa as estimated in SDS-PAGE under reducing conditions.

Human EGF protein measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. (Rubin, J.S. et al. 1991, Proc. Natl. Acad. Sci. USA 88:415.) The ED50 for this effect is typically 50-200 pg/mL.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.