The recombinant human Noggin protein is expressed in Human Cells, whose amino acid sequence is derived from human NOG (NP_005441.1), (Met1-Cys232) was expressed with five amino acids (DDDDK) at the C-terminus.

The recombinant human NOG consists 211 amino acids and predicts a molecular mass of 23.7 kDa.

Human Noggin protein measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.18-0.7 ug/mL in the presence of 50 ng/mL of hBMP4.

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