The recombinant human IL-1 alpha protein is expressed in Human Cells, whose amino acid sequence is derived from the processed form of human IL1α (NP_000566.3), (Ser 113-Ala 271) was expressed and purified.

The recombinant human IL1α consists of 159 amino acids and has a predicted molecular mass of 18 kDa. As a result of glycosylation, the apparent molecular mass of rhIL1α is approximately 22 kDa in SDS-PAGE under reducing conditions.

Human IL-1 alpha protein measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 0.2-1 ng/mL .

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