The recombinant human bFGF protein is expressed in E. coli, whose amino acid sequence is derived from mature form of human bFGF (NP_001997.5), (Pro 143-Ser 288) was expressed, with an additional Met at the N-terminus.

The recombinant human bFGF is a disulfide-linked homodimeric protein. The reduced monomer consists of 147 amino acids and has a calculated molecular mass of 16.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of bFGF is approximately 17 kDa.

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