ChIP Validated Antibody Primer Sets with positive and negative gene targets

Chromatrap^® ChIP-validated Antibodies can now be supplied with the correct matching primer sets to cover all aspects of ChIP validation.

 •Reduce time spent searching databases and designing forward and reverse primers
 •Purchase superior quality ChIP-validated antibodies with their matching high-quality primers
 •Have confidence that the primers are tested with the exact antibody and gene target

Many common epigenetic targets of interest are now available as combined antibody & primer sets from Chromatrap^®.

ChIP Validated Sin3a Antibody with Positive and Negative Primer Sets
Product Code : 900007

Chromatrap^®’s ChIP Validated Sin3a Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: Sin3a antibody, control sheep IgG, positive and negative primer sets. The ChIP Validated Sin3a Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.

Background:

Sin3a (Switch independent 3 homologue) is a transcriptional repressor and protein-encoding gene. It interacts with co-repressors and DNA-binding proteins such as REST, MXl1 and MXD1-MAX that allow Sin3a to bind to DNA and repress transcriptional activity. It acts interdependently with OGT (O-linked N-acetylglucosamine) to repress transcription alongside with histone deacetylation.

A sheep IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.

The Sin3a positive primer set recognises the hypermethylated promoter of a gene that binds to Sin3a, resulting in the repression of transcription. The negative gene target included recognises a gene that is associated with active transcription.

•Applications: ChIP
•Source: Sheep
•Concentration: 1mg/ml
•Type: Monoclonal
•Size: 50μl
•Purification: Protein G (affinity purified)
•Specificity: Human
•Storage Conditions: The Sin3a antibody and sheep IgG +4°C. Primer sets -20°C.

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For further information, including custom built microplates, reagent reservoirs, vacuum manifold design or microplate handling equipment, please contact us: Tel: +44 1978 666240; Email: int.sales@porvair-sciences.com.