ChIP Validated Antibody Primer Sets with positive and negative gene targets

Chromatrap^® ChIP-validated Antibodies can now be supplied with the correct matching primer sets to cover all aspects of ChIP validation.

•Reduce time spent searching databases and designing forward and reverse primers
•Purchase superior quality ChIP-validated antibodies with their matching high-quality primers
•Have confidence that the primers are tested with the exact antibody and gene target

Many common epigenetic targets of interest are now available as combined antibody & primer sets from Chromatrap^®.

ChIP Validated H3K9me3 Antibody with Positive and Negative Primer Sets
Product Code : 900005

Chromatrap^®’s ChIP Validated H3K9me3 Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: H3K9me3 antibody, control mouse IgG, positive and negative primer sets. The ChIP Validated H3K9me3 Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.

Background:

Histone 3 tri methyl K9 (H3K9me3) is a histone mark associated with repressed chromatin. It condenses and compacts the chromatin, restricting the transcription machinery from binding and carrying out gene expression. It is a marker of constitutive heterochromatin, and generally associated with gene silencing. As a histone mark, it is widely found throughout human cell lines, serving as an abundant antibody target for ChIP.

A mouse IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.

H3K9me3 is usually associated with closed chromatin and repressive transcription. The positive primer set included in this Antibody Primer Set recognises a protein-coding gene that is present when transcription is switched off. The negative gene target included recognises a gene that is associated with open chromatin and active transcription at multiple loci in the genome.

•Applications: ChIP
•Source: Mouse
•Concentration: 1mg/ml
•Type: Monoclonal
•Size: 50μl
•Purification: Protein A (affinity purified)
•Specificity: Human
•Storage Conditions: -20°C

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