Features
• Non-PCR inhibiting intercalating dye
• Rapid extension rate for early Ct values
• Market leading sensitivity - increased limit of detection
• Compatible on all real-time PCR platforms - standard and fast cycling conditions
PCR Biosystems use a proprietary intercalating dye which does not inhibit PCR, unlike other popular fluorescent dyes including SYBR® Green. Combined with advanced enzyme, hot-start and reaction buffer technology we offer market leading sensitivity and reproducibility.
qPCRBIO SyGreen Mix can be used to quantify any DNA template including genomic, cDNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. Proprietary small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer market leading performance with minimal or no optimisation.
Many modern real-time PCR instruments have a fast cycling mode. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen Mix in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.
We have compared the performance of our mix and 6 competitor mixes on 4 house keeping genes, selected at random from the RTPrimerDB qPCR primer database. qPCR performance was assessed in terms of Ct values, sensitivity and efficiency. Overall, qPCRBIO SyGreen Mix outperformed all 6 competitor mixes tested. Click here to download the results of this detailed competitor comparison.
For which ROX variant is compatible with your real-time PCR instrument, see our selection table.
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PCR Biosystems offers a range of best-in-class kits and reagents for PCR and related technology. By combining our enhanced polymerases with highly-developed reaction buffers and novel hot-start chemistry PCR Biosystems is leading the development of PCR. Our PCR reagents have been developed to maximise yield and sensitivity in the simplest and most challenging of reactions.
Our portfolio covers a broad range of applications including, real-time PCR, endpoint PCR, high fidelity PCR, hot-start PCR, long PCR, PCR direct from crude samples and molecular diagnostic PCR.
All PCR Biosystems products have undergone comprehensive competitor product comparisons. On average we out perform all competitors in yield, specificity, sensitivity and speed. For more information on our technology click here.
PCR Biosystems produces, manufactures and distributes under a comprehensive quality management system, following ISO 9001:2008 standards.
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