Catalog #	Pack size	Price(€)
ZP02033	200U	55.00
ZP02034	500U	120.00

Description:
DNA polymerase from Thermococcus kodakaraensis KOD is one of the most efficient thermostable PCR enzymes exhibiting higher accuracy and elongation velocity than any other commercially available DNA polymerase.The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5''---->3'' direction. The KOD DNA Polymerase also exhibits 3''--->5''exonuclease (proofreading) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5''--->3'' exonuclease activity.
Concentration:5U/ul

Source:
KOD DNA Polymerase (native) - Thermococcus kodakaraensis cells.KOD DNA Polymerase (recombinant) - E.coli cells with a cloned pol gene from Thermococcus kodakaraensis.
10X Reaction Buffer with MgCl2:
1 ml 10X Buffer : 1.2 M Tris-HCl, 100 mM KCl, 60 mM (NH4)2SO4, 25 mM MgCl2, 1% Triton X-100, 0.01% BSA, pH8.0 .
Features:

High fidelity than other normal DNA polymerase
Higher processivity.
Higher yield in short time

Applications:
All PCR* amplification which demands high fidelity.
Primer extension.
High fidelity PCR for cloning into blunt-ended vectors.
Site-directed mutagenesis.

Unit Definition:
One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol dNTP into acid insoluble form in 30 minutes at 75°C in a reaction containing 20 mM Tris-HCl (pH7.5 at 25°C), 8 mM MgCl2, 0.5 mM DTT, 50ug/ml BSA, 150 uM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]-dTTP) and 150 ug/ml activated calf thymus DNA.

Quality Control Tests:
PCR (activity), SDS-PAGE (purity), endonuclease/nickase.

Storage:
5U/ul in 50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.1% Nonidet P-40, 0.1% Tween-20, pH8.0

ShineGene Bio-Technologies, Inc., located in Shanghai, P.R. China, is a bio-company specialized in providing bio- technical services including Gene Synthesis,Peptide Synthesis,Real Time PCR, Fluorogenic Probe Design,Fluorogenic Probe Label,HRP Enzyme Stabilizer,X-gal,IPTG,ProClin300etc. and supplying reagents such as Hotstart Taq polymerase, dNTPs, qPCR mastermix,one step qPCR kits,First Strand cDNA Synthesis Kits,etc. ShineGene also undertake some OEM or OED orders as well as production task.ShineGene works at the cutting edge of development in PCR, quantitative real-time PCR, transposon and RNA technologies. ShineGenes mission is to provide new tools of excellent quality and extreme purity to the scientific community. Therefore research and development is a continuous process in our company.

http://www.synthesisgene.com
http://www.shinegene.org.cn/englsih