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Paraffin-embedded Tissue Sections MicroRNA Extraction Kit (Spin-column)
Paraffin-embedded Tissue Sections MicroRNA Extraction Kit (Spin-column)
Origin of place China
Model 50/100
Supplier BioTeke Corporation
Price $800/$1500
Hits 1135
Updated 11/2/2018
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Paraffin-embedded Tissue Sections MicroRNA Extraction Kit (Spin-column)
 

Cat.#
Preps
Price(USD)
RP 5311
50
800
RP 5312
100
1500


 

Principle
The RNApure procedure represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica membrane with the speed of microspin technology. A specialized high-salt buffer system allows up to 100 μg of RNA longer than 200 bases to bind to the RNApure silica membrane. Biological samples are first lysed and homogenized in the presence of a highly denaturing guanidine-thiocyanate–containing buffer, which immediately inactivates RNases to ensure intact RNA. Ethanol is added to provide appropriate binding conditions, and the sample is then applied to an RNApure spin-column, where the total RNA binds to the membrane and contaminants are efficiently removed. High-quality RNA is then eluted in 30–100 μl water.

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