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BCA Protein Assay Kit
BCA Protein Assay Kit
Origin of place China
Model 200/500/2500
Supplier BioTeke Corporation
Price $36/$72/$305
Hits 1476
Updated 11/2/2018
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BCA Protein Assay Kit
 

Cat.#
Preps
Prices(USD)
PP1001
200
36
PP1002
500
72
PP1003
2500
305


Description:
The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid. Two molecules of BCA chelate with one cuprous ion forming purple-coloured product. The BCA/copper complex is water-soluble and exhibits a strong absorbance at 562nm. Protein concentration can be determined based on absorbance because of the linear relation between them.

Features:
 

  • Faster and easier than the Lowry method
  • Sensitive, The minimum detection level of 25μg/ml.
  • Compatible with most detergents(SDS/TritonX-100/ Tween20/Tween60/Tween80 up to 5%).
  • Good linear range from 20-2000μg/ml.
  • Coefficient of variation lower than Bradford. 
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