RNzol LS Reagent: Blood/Liquid Sample Total RNA Extraction Kit(Spin-column)

 

Cat.#	Preps	Price（USD）
RP1101	50ml	70
RP1102	100ml	130

 

Description

RNzol LS Reagent is a ready-to-use reagent for the isolation of total RNA from whole blood and other liquid samples. The reagent, a mono-phasic solution of phenol and guanidine isothiocyanate, is an improvement to the single-step RNA isolation method developed by Chomczynski and Sacchi. During sample homogenization or lysis, RNzol LS Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation.

Total RNA isolated by RNzol LS Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly(A)+ selection, in vitro translation, RNase protection assay, and molecular cloning. For use in the polymerase chain reaction (PCR), treatment of the isolated RNA with amplification grade DNase I is recommended when the two primers lie within a single exon. 

RNzol LS Reagent facilitates isolation of a variety of RNA species of large or small molecular size. For example, RNA isolated from rat liver, electrophoresed on an agarose gel, and stained with ethidium bromide, shows discrete bands of high molecular weight RNA between 7 kb and 15 kb in size, (composed of mRNA’s and hnRNA’s) two predominant ribosomal RNA bands at ~5 kb (28S) and at ~2 kb (18S), and low molecular weight RNA between 0.1 and 0.3 kb (tRNA, 5S). The isolated RNA has an A260/A280 ratio ≥1.8 when diluted into TE.


Features

◆Convenient, red cell and while cell do not need to be separated, additional red cell lysis procedure is cut
◆Rapid, easy-to-follow， high-quality RNA can be harvested within 30min
 

BIOTEKE is a leading biotechnology company in China since 2005,
Our products nucleic acid extraction/purification as well as related essential lab instruments.
Please check our website here: http://english.bioteke.cn/

Our main product portfolio includes but not limits the following items,
Reagents/kits for molecular biological research uses,
DNA/RNA/plasmid extraction kits, PCR/RT-PCR/qPCR products, DNA/RNA stains/markers.

Automated Nucleic Acid Extraction systems, together with it's magnetic beeds kits;
For extracting DNA/RNA from various samples(cfDNA, blood, tissue/cells, plants, plasmid, etc) ,
32 samples or 96 samples in one time in 30min,
The cost for one samples is less than 4USD for our clients.

Micro Spectrophotometer ND5000,
0.5-2uL sample is needed, 4s for 1 test, to test the concentration of DNA/RNA/proteins.

Storage tubes for blood RNA, cfDNA, Urine DNA, swab/saliva DNA, stool DNA samples.

samples can be transported for more than one week, or for long term storage in low temprature.
widely used in clinical sample collections.Trial samples are available.

Our R