Human Mesenchymal Stem Cell Serum-free Medium (HMSC-SFM)
★ Technical Manual
Human Mesenchymal Stem Cell Serum-free Medium (HMSC-SFM)
★ Catalog NO: BW110020
★ Components
490 ml of Basal Medium
5 ml of Human Mesenchymal Stem Cell Supplement (HMSCS)
5 ml of penicillin/streptomycin solution (P/S)
★ Product Description
Human Mesenchymal Stem Cell Serum-free Medium (HMSC-SFM) contains defined components including essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and other supplements. The product is suitable for culturing human mesenchymal stem cells. The product is strictly aseptic without any contamination of virus or mycoplasma, and exhibits stable performance. By using this medium, human mesenchymal stem cells can be quickly expanded with minimal differentiation.
★ Instruction for Use
It is strongly recommended that cell plates should be precoated with CELLstart™1:50 (Cat. No. A10142-01, GIBCO) in Dulbecco’s Phosphate Buffered Saline with calcium and magnesium (Cat. No. A12858, GIBCO).
1. Dilute CELLstart™1:50 (Cat. No. A10142-01, GIBCO) in Dulbecco’s Phosphate Buffered Saline with calcium and magnesium (Cat. No. A12858, GIBCO).
2. Add diluted CELLstart™ to culture container at a final volume per surface area of 0.078 mL/cm2. Refer to table for respective culture container:
Culture Container Surface Area (cm2) Volume of 1:50 Diluted CELLstart
6-well plate 9.6 750ul per well
12-well plate 3.2 250ul per well
24-well plate 2.0 160ul per well
3. Incubate in a 37°C, 5% CO2 incubator for 2 hours. It is recommended to coat culture containers the day of use or the day before. If precoating the day before, the culture container must be stored at 2 to 8°C wrapped with Parafilm® to avoid drying.
4. Pipette out diluted CELLstart™ from culture container and discard. Culture vessel is ready for the addition of cells.
*Note: It is not necessary to rinse the culture container after removal of CELLstart™. The bottom of the coated culture container should have a clear and wet appearance.
5. Thaw penicillin/streptomycin solution (P/S) overnight at 2 to 8 ℃.
6. Add the above thawed solutions to the Basal Medium, gently swirl the bottle to ensure the mixture homogeneity.
7. Thaw the Human Mensenchymal Stem Cell Supplement (HMSCS) in water bath (37℃) and then add it into the above mixture to make the complete medium. 8. Store the complete medium in the dark at 4℃.
★ Product Use
This product is for laboratory research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
★ Storage Condition
Basal Medium should be stored at 4℃, while HMSCS and P/S solution should be stored in the dark at -20℃.
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Biowit Technologies Ltd. is a high-tech company specialized in providing R&D services and related products in the biological field. As a technology-driven and customer-focused company, BioWit provides a broad and integrated service and product portfolio including molecular biology, virus packaging (e.g., adenovirus, AAV, lentivirus, retrovirus and baculovirus), protein expression, cell biology, stem cells and transgenic models. One of the milestones achieved by Biowit is its recent sucess in the development of a series of serum-containing media as well as serum-free or animal -free media for stem cell culture, which are tailored for clinical applications. The core technology team members have engaged in research and development for many years in world-famous academic institutions or prestigious drug companies. Biowit has established collaboration with international prestigious research institutions. The worldwide collaboration enables Biowit to timely renew its biotechnologies (http://www.biowit.com).
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