Working Principle of Real-time PCRs
Fluorescence quantitative PCR technique is a kind of analyzing method that to add expert probes marked with fluorescent group into PCR reaction solution, and during the PCR process, owing to the hydrolyzation of the fluorescence probe by Taq polymerase, fluorescence signals will be accumulated so to realize real-time monitoringthe whole PCR course and finally by comparing to the standard curve to realize the quantitative analysis of unknowntemplate. Here, our fluorescence probes adopt the latest technique of Taqman, "TaqmanMGB", which has two main advantages compared to the traditional Taqman probe:
① Probe 3 × is marked with fluorescent quencher that can't illuminate by itself in stead of common illuminant Tamara fluorescence label. This new technique can reduce the fluorescence background and improve resolving power of fluorescence spectrum, especially improve the resolving power among fluorescence spectrums of different reportersduring SNP analysis.
② Probe 3 × combines Minor groove binder so as to increase the Tm value and strengthen the hybridization stability.

Features of Taqman MGB
● Low fluorescence background and high sensitivity.
● Enlarge the difference of Tm values between target template and various non-specific strips as same as the specificity.
● New fluorescence Taqman MGB probe can improve hybridization stability with higher resolving power compared to traditional Taqman probe.
● Long-life storage.
● Good experimental repetition. 　

Pathogeny Real-time PCR detection kits

S/N Names Manufacturer Packing BSJ010120001L00 HBV Real-time PCR detection kits BIOER 96T/kit BSJ010220001L00 HCV Real-time PCR detection kits BIOER 96T/kit

Cancers Real-time PCR reaction mix

S/N Name Manufacturer Packing BSJ012320001L80 GAPDH Real-time PCR reaction mix BIOER 96T/kit BSJ012420001L80 MMP-2 Real-time PCR reaction mix BIOER 96T/kit BSJ012520001L80 TIMP-2 Real-time PCR reaction mix BIOER 96T/kit BSJ012620001L80 VEGF Real-time PCR reaction mix BIOER 96T/kit BSJ012720001L80 KDR Real-time PCR reaction mix BIOER 96T/kit BSJ012820001L80 FLT-1 Real-time PCR reaction mix BIOER 96T/kit BSJ012920001L80 EGF Real-time PCR reaction mix BIOER 96T/kit BSJ013020001L80 COX-2 Real-time PCR reaction mix BIOER 96T/kit BSJ013120001L80 BFGF Real-time PCR reaction mix BIOER 96T/kit BSJ013220001L80 hTERT Real-time PCR reaction mix BIOER 96T/kit BSJ013320001L80 HTR Real-time PCR reaction mix BIOER 96T/kit BSJ013420001L80 EGFR Real-time PCR reaction mix BIOER 96T/kit BSJ013520001L80 TNF Real-time PCR reaction mix BIOER 96T/kit BSJ013620001L80 TGF-a Real-time PCR reaction mix BIOER 96T/kit BSJ013720001L80 TGF-b Real-time PCR reaction mix BIOER 96T/kit Notes: For the reaction mix, we supply primer and probes.

PCR Related Reagents

S/N Name Manufacturer Packing Remarks BSJ000120001S40 Top-Taq Polymerase Imported 200U   BSJ000120001L40 Top-Taq polymerase Imported 500U   BSJ000120001S40 pfu polymerase Imported 100U High fidelity Taq polymerase BSJ000120001M40 pfu polymerase Imported 250U High fidelity Taq polymerase BSJ000220001M80 SYBR MIX BIOER 100 test

GMO products PCR detection reagents (Primer & Probes)

S/N Name Manufacturer Packing Remarks BSJ02012001S40 GMO-1 PCR kit BIOER 96T/kit To detect 35S start-up point BSJ020220001L40 GMO-2 PCR kit BIOER 96T/kit To detect 18S gene BSJ020320001S40 GMO-3 PCR kit BIOER 96T/kit

As a Japanese wholly owned enterprise, BIOER TECHNOLOGY CO., LTD. is the manufacturing base of the Bio Flux Corporation whose headquarter lies in Tokyo, Japan. BIOER is committed to researching and manufacturing of hi-tech products for life science applications. Years of experience and technical know-how have been developed into three product lines： life science instrumentation, related reagents and disposables and general laboratory instrumentation,such as Real-time PCR systems, thermal cyclers, cooling & heating blocks,Nucleic Acid Purification Systems, HBV, HCV, HIV, GMO, nucleic acid purification kits and all kinds of constant temperature baths. These products are recognized in both the overseas and domestic markets and have won praise from customers all over the world, including: Hong Kong, Taiwan, Korea, Japan, India, Malaysia & USA.

BIOER moved to its new state of the art facility in September 2003 that occupies a floor space of 16,000 square meters . BIOER uses the latest computer technology throughout its production process and strictly follows international ISO 13485 standards aiming at creating a first-class brand and products with first-class after-sales services for its clients.