The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands.  The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

 

Storage Buffer : 
20 mM Tris-phosphate(pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.

Quality Control : 
Under suggested conditions, Unveil Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.

Storage :
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at  -20°C.

Note. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.

All products are for research use only.

Caution :
Not intended for human or animal diagnostic or therapeutic uses.

Bio-Helix has been focusing on providing the best bio-reagents for life science researchers all over the world since 2007. Our products, to name a few, include DNA markers, protein markers, staining reagents, nucleic acid purification, PCR reagents and so on.

Working together with scientists in the lab around the world and continuing efforts of quality improvement, we begin to gain trusts and appraises from customers. The brand, Bio-Helix, spreading in more than forty countries, has become a trustworthy and welcome brand of reagents in laboratories of life science.

With the expansion of product lines as well as customers, we discover a greater need for a good quality supply of bio-reagents from the medical circle. Mostly, these needs are for diagnostic products. The demand of good quality bio-reagents for diagnostic devices opens a whole new possibility of innovation and motivation for Bio-Helix.

We have a vision that one day our life span can be elongated; life quality can be improved by early detection and subsequent proper treatment of all diseases. Ancient Chinese believed that a good doctor treats the disease before illness, instead of after its onset. In Bio-Helix, a new brand, LifeDirex, is created to fulfill this vision. LifeDirex will be focusing on supplying the best and most innovative reagents for diagnostic tools to promote the earliest detection of diseases as possible.

Our mission is to provide the best quality products and to develop novel technologies that assist researchers to find solutions in quest of scientific achievements. Furthermore, we decide now is the best time to enter the market of in vitro diagnostic device (IVDD). It is our goal to become the best solution provider for both research laboratory and IVDD.

Driven by desires to improve life quality and achieve scientific excellence, we operate with the highest standards of integrity. We also extend the spirit to our services and global value to our customers all around the world. A diverse workforce and network of collaboration from a great mix of company culture. Bio-Helix is not only a company of equal opportunity but also works hard to provide a friendly and growing environment.