The Blu10 Prestained Protein Ladder (BlueRAY) is a combination of 10 pre-stained proteins with molecular weights from 11 to 180 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa, serve as reference bands. The Blu10 Prestained Protein Ladder (BlueRAY) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size, and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

 

-Guide for Molecular Weight Estimation (kDa)

Migration patterns of Blu10 Prestained Protein Ladder (BlueRAY) in different electrophoresis conditions are listed below :

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

 

Bio-Helix has been focusing on providing the best bio-reagents for life science researchers all over the world since 2007. Our products, to name a few, include DNA markers, protein markers, staining reagents, nucleic acid purification, PCR reagents and so on.

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