Description

The blood sample contains a large number of red blood cells and platelet cells, which makes blood processing more troublesome, and the purity and quantity of blood genomic DNA purified by conventional methods is low. The kit uses Foregene Protease Plus and unique BL1 and BL2 buffer systems, so anticoagulated blood and coagulated blood samples can be completely digested in a short time, thereby minimizing DNA degradation and obtaining the maximum amount of genomic DNA. The fast blood processing system and simple spin column operation greatly simplify the purification of blood genome, and high-quality and high-purity genomic DNA can be obtained within 40 minutes.

The DNA-only silica gel membrane used in the spin column of the kit is Foregene's unique new material, which can efficiently and specifically bind DNA, and can remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The maximum capacity of the spin column is 80 μg DNA. The obtained DNA can be used in molecular biology experiments, such as restriction digestion, PCR, Southern hybridization and DNA library construction.

Specification

Kit contents	DE-05111	DE-05112	DE-05113
	50 T	100 T	250 T
Buffer BL1	15 mL	30 ml	75 ml
Buffer BL2 *	15 mL	30 ml	75 ml
Buffer DC	100 mL	200 ml	500 ml
Buffer PW *	25 mL	50 ml	125 ml
Buffer WB1	15 mL	30 ml	75 ml
Buffer EB	10 mL	20 ml	50 ml
Foregene Protease Plus	1.25 mL	2.5 ml	6.5 ml
DNA-Only Column	50	100	250
Manual	1	1	1

*: Buffer BL2 and Buffer PW contain irritating liquid salt. Please wear gloves and take relevant protective measures when operating.

Features&advantages

-No RNase contamination: The DNA-Only Column provided in the kit makes it possible to remove RNA from genomic DNA without additional RNase during the experiment, thereby preventing the laboratory from being contaminated by exogenous RNase.

-Fast speed: Foregene Protease has higher activity than similar proteases and digests tissue samples faster;

-Simple: the genomic DNA purification operation can be completed in 50 minutes.

-Convenient: The centrifugation is performed at room temperature, 4℃ centrifugation and ethanol precipitation of DNA is not required.

-Safety: no organic reagent is used.

-High quality: The purified genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

Kit application

It is suitable for extraction and purification of genomic DNA from fresh or frozen anticoagulated whole blood.

Storage and Shelf life

The kit can be stored for 12 months at room temperature (15-25℃), or 2-8℃ for longer time.

Foregene Co., Ltd. is specialized in the molecular biology lab reagents, IVD raw materials, IVD Kits, and related products and services.Foregene is integrated in R&D, manufacturing and marketing together .

Established in 2011, Foregene has owned the world-class Direct PCR Platform,DNA-Only,RNA double spin column Isolation Platform. The main products include Direct PCR reagents, DNA/RNA isolation kits, PCR Master Mix, Genotyping kits, FISH Probe Kits, IVD Raw Materials, various IVD kits, and Lab Equipments. Foregene can provide the whole solution of IVD products based on our technology Platforms.

Becuse of the world-class leading technology, we are popular in domestic and overseas markets.Foregene’s high quality products are widely used in hospitals, universities, national research institutions, medical laboratories,CRO companies, bio-pharm companies, etc.

Looking forward to Cooperate with you.