Description

This kit uses the spin column and formula developed by our company, which can efficiently extract small fragments of RNA such as 20-200nt miRNA, siRNA, snRNA from various animal tissues and cells. The kit provides DNA-Cleaning Column, which can easily separate the supernatant from the tissue lysate and remove DNA and long RNA from the sample, which is simple and time-saving; RNA-only Column can efficiently bind RNA with a unique formula , Can process a large number of samples at the same time. The whole system is RNase-Free, so that the purified miRNA is not degraded; Buffer miRW1, Buffer miRW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ions, and organic compound pollution.

Specification

Kit composition	RE-0101T	RE-01011	RE-01012	RE-01014
	5 T	50 T	100 T	200 T
Buffer miRL*	3ml	25ml	50ml	100ml
Buffer miRW1	4ml	35ml	70ml	140ml
Buffer miRW2	2.4ml	24ml	48ml	96ml
RNase-Free ddH2O	1.7ml	10ml	20ml	40ml
RNA-only Column	5	50	100	200
DNA-Cleaning Column	5	50	100	200
IFU	1	1	1	1

*Please wear gloves and take protective measures during the operation as Buffer RL1 contains irritating chaotropic salts.

Features

◆ The whole process is operated at room temperature (15-25°C), without ice bath and low-temperature centrifugation.

◆ Full set kit RNase-Free, no need to worry about RNA degradation.

◆ DNA-Cleaning Column specifically binds DNA, so that the kit can remove genomic DNA contamination without adding additional DNase.

◆ High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.

◆ Fast speed: The operation is simple and can be completed within 30 minutes.

◆ Safety: no organic reagent extraction is required.

◆ High quality: The extracted RNA fragments are of high purity, free of protein and other impurities pollution, and can meet the requirements of downstream

◆ Various experimental applications

Kit Application

It is suitable for the extraction and purification of miRNA from a variety of fresh or frozen animal tissues or cells.

Storage conditions

This kit can be stored for 24 months under dry conditions at room temperature (15-25°C), if it needs to be stored for a longer period of time, it can be stored at 2-8°C. Buffer miRL can be placed at 4°C for 1 month after adding β-mercaptoethanol (it is recommended to add it now).

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.

Foregene Co., Ltd. is specialized in the molecular biology lab reagents, IVD raw materials, IVD Kits, and related products and services.Foregene is integrated in R&D, manufacturing and marketing together .

Established in 2011, Foregene has owned the world-class Direct PCR Platform,DNA-Only,RNA double spin column Isolation Platform. The main products include Direct PCR reagents, DNA/RNA isolation kits, PCR Master Mix, Genotyping kits, FISH Probe Kits, IVD Raw Materials, various IVD kits, and Lab Equipments. Foregene can provide the whole solution of IVD products based on our technology Platforms.

Becuse of the world-class leading technology, we are popular in domestic and overseas markets.Foregene’s high quality products are widely used in hospitals, universities, national research institutions, medical laboratories,CRO companies, bio-pharm companies, etc.

Looking forward to Cooperate with you.