Species-specific positive probes and negative control probes are needed to run with human or mouse control slides for a quality control check of the technique and with your sample for the sample or RNA quality control check.

ACD provides positive control probes that target species-specific housekeeping genes with different expression levels.  Negative control probes target the bacteria DapB gene.

Careful selection of the right positive control is strongly recommended.  The expected expression level of your target RNA is the key criteria to consider as your positive control probe should have similar expression profile. See the overview page for details.

The negative and positive control probes in channel 1 can be used for both RNAscope® 2.5 HD BROWN or RNAscope® 2.5 HD RED assay, as both assays function in Probe Channel 1. The names for these probes do not contain a C1 or Channel 1 in its names.

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Bio-Techne Corporation (NASDAQ: TECH) is a leading developer and manufacturer of high quality purified proteins:notably cytokines and growth factors, antibodies, immunoassays, as well as biologically active small molecule compounds and ACD's in situ hybridization detection products,which are sold to biomedical researchers and clinical research laboratories; these operations constitute the core Biotechnology Division, headquartered in Minneapolis, Minnesota. The Protein Platforms Division manufactures innovative protein analysis tools under the ProteinSimple brand name that greatly automate western blotting and immunoassay practices. The Diagnostics Division manufactures FDA-regulated controls, calibrators, blood gas and clinical chemistry controls and other reagents for OEM customer and clinical customers. Bio-Techne products are integral components of scientific investigations into biological processes and the nature and progress of specific diseases. They aid in drug discovery efforts and provide the means for accurate clinical tests and diagnoses. With thousands of products in its portfolio, Bio-Techne generated approximately $563 million in net sales in fiscal 2017 and has approximately 1,800 employees worldwide. For more information on Bio-Techne and its brands, please visit www.bio-techne.com.