The RNAscope® 2.5 VS Assay is designed as an automated assay on the Discovery Ultra and Discovery XT Automated IHC/ISH slide staining systems from Roche. The RNAscope® 2.5 VS Assay, is an RNA in situ hybridization assay based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope® Assays do not require the RNA-free environment used for traditional ISH.

RNAscope® 2.5 VS Reagent Kit-BROWN is a very robust assay gives reliable results which are ideal for archiving due to the permanent staining. It is the standard in molecular pathology diagnostics, usable for a wide source of samples and the results are visible under any standard bright field microscope. The RNAscope® 2.5 VS Reagent Kit—BROWN  is designed to provide more intense Diaminobenzidine (DAB) staining—ideal when low copy target gene expression is anticipated (1–20 copies per cell). The stained slides are visualized with brightfield microscopes.

The RNAscope® 2.5 VS Reagent Kit-RED with it Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed,  ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope.

These new RNAscope® 2.5 VS reagents required the new RNAscope® 2.5 VS Probes for optimal performance. Probe designs are identical to previous version, however the probes are manufactured for optimized performance with RNAscope® 2.5 reagent chemistry.

Sample Images



RNAscope® VS Automated Assay - BROWN workflow Requires the following from ACD:

RNAscope® 2.5 VS Target Probes (Catalog or Made-to-Order VS Probes)
RNAscope® 2.5 VS Control Probes (Select species-specific VS positive control probes or packs of probes and VS negative control probes)
RNAscope® Control Slides (optional)
RNAscope® 2.5 VS Assay Reagent Kit—BROWN

RNASCOPE® 2.5 VS Automated Assay - Red workflow Requires the following from ACD:

RNAscope®  2.5 VS Target Probes (Catalog or Made-to-Order VS Probes)
RNAscope®  2.5 VS Control Probes (Select species-specific VS positive control probes or packs of probes and VS negative control probes)
RNAscope® Control Slides (optional)
RNAscope® 2.5 VS  Reagent Kit—RED

Bio-Techne Corporation (NASDAQ: TECH) is a leading developer and manufacturer of high quality purified proteins:notably cytokines and growth factors, antibodies, immunoassays, as well as biologically active small molecule compounds and ACD's in situ hybridization detection products,which are sold to biomedical researchers and clinical research laboratories; these operations constitute the core Biotechnology Division, headquartered in Minneapolis, Minnesota. The Protein Platforms Division manufactures innovative protein analysis tools under the ProteinSimple brand name that greatly automate western blotting and immunoassay practices. The Diagnostics Division manufactures FDA-regulated controls, calibrators, blood gas and clinical chemistry controls and other reagents for OEM customer and clinical customers. Bio-Techne products are integral components of scientific investigations into biological processes and the nature and progress of specific diseases. They aid in drug discovery efforts and provide the means for accurate clinical tests and diagnoses. With thousands of products in its portfolio, Bio-Techne generated approximately $563 million in net sales in fiscal 2017 and has approximately 1,800 employees worldwide. For more information on Bio-Techne and its brands, please visit www.bio-techne.com.