Directed stem cell reprogramming using synthetic mRNA
 

amsbio now offers a non-integrating strategy for reprogramming stem cell fate based on the administration of synthetic modified mRNA that greatly increases efficiency whilst reducing the innate antiviral response of viral protocols.

 

Advantages of synthetic mRNA

• Pre-made, highly purified and ready-to-use
• Simple, safe, non-integrating technique
• Modified to overcome innate antiviral responses
• Reprogram multiple human cell types to pluripotency
• Higher Efficiencies than traditional protocols
• Direct cell fate and transdifferentiation

 

The method for deriving iPS cells originally described by Yamanaka remains the most established technique. However, several barriers have hampered the wide-spread applicability of iPS cells using this technique. Traditional methods used retrovirus transduction of the reprogramming factors which results in viral integration into the genome and has low reprogramming efficiencies. Viral integration and low efficiency presents downstream problems to the therapeutic use of iPS cells. Synthetic highly modified mRNA overcomes these obstacles by inducing pluripotency without incurring genetic change. Messenger RNA (mRNA) is the key intermediary in gene expression, translating the DNA's genetic code into the amino acids that make up proteins. The direct link of mRNA to protein makes this technique ideally suited for nuclear reprogramming. The high efficiency of synthetic mRNA based reprogramming was recently reported in Cell Stem Cell (Warren, 2010). In addition, synthetic mRNA is a powerful tool to direct stem cell fate and transdifferentiation.

Latest Publication:

 Ilic D et al. (2014) 3D In Vitro Model of a Functional Epidermal Permeability Barrier from Human Embryonic Stem Cells and Induced Pluripotent Stem Cellss. Stem Cell Reports Vol. 2, 1-15

 

 

Synthetic mRNA is the most advanced and safest method for deriving your iPS cells. amsbio offers ready-to-order synthetic modified mRNA proven to reprogram a wide variety of human cell types. Alternatively made-to-order custom mRNA is available to meet unique requirements.

 

Description	Catalog Number	Pack Size	Order
Premixed mRNA set for reprogramming - KMOSL	7001-100M-20ug	20 ug
Premixed mRNA set for reprogramming - KMOSL	7001-100M-200ug	200 ug
Premixed mRNA set for reprogramming - KMOSL	7001-100M-500ug	500 ug
Premixed mRNA set for reprogramming - KMOSL	7001-100M-1mg	1 mg
Oct4 mRNA, human	7001-101-20ug	20 ug
Oct4 mRNA, human	7001-101-200ug	200 ug
Oct4 mRNA, human	7001-101-500ug	500 ug
Oct4 mRNA, human	7001-101-1mg	1 mg
Klf4 mRNA, human	7001-102-20ug	20 ug
Klf4 mRNA, human	7001-102-200ug	200 ug
Klf4 mRNA, human	7001-102-500ug	500 ug
Klf4 mRNA, human	7001-102-1mg	1 mg
Sox2 mRNA, human	7001-103-20ug	20 ug
Sox2 mRNA, human	7001-103-200ug	200 ug
Sox2 mRNA, human	7001-103-500ug	500 ug
Sox2 mRNA, human	7001-103-1mg	1 mg
c-Myc mRNA, human	7001-104-20ug	20 ug
c-Myc mRNA, human	7001-104-200ug	200 ug
c-Myc mRNA, human	7001-104-500ug	500 ug
c-Myc mRNA, human	7001-104-1mg	1 mg
Lin-28 mRNA, human	7001-105-20ug	20 ug
Lin-28 mRNA, human	7001-105-200ug	200 ug
Lin-28 mRNA, human	7001-105-500ug	500 ug
Lin-28 mRNA, human	7001-105-1mg	1 mg
nGFP mRNA	7001-201-20ug	20 ug
nGFP mRNA	7001-201-200ug	200 ug
nGFP mRNA	7001-201-500ug	500 ug
nGFP mRNA	7001-201-1mg	1 mg
EGFP mRNA	7001-202-20ug	20 ug
EGFP mRNA	7001-202-200ug	200 ug
EGFP mRNA	7001-202-500ug	500 ug
EGFP mRNA	7001-202-1mg	1 mg
Firefly Luciferase (FLuc) mRNA	7001-203-20ug	20 ug
Firefly Luciferase (FLuc) mRNA	7001-203-200ug	200 ug
Firefly Luciferase (FLuc) mRNA	7001-203-500ug	500 ug
Firefly Luciferase (FLuc) mRNA	7001-203-1mg	1 mg
EPO mRNA, human	7001-106-20ug	20 ug
EPO mRNA, human	7001-106-200ug	200 ug
EPO mRNA, human	7001-106-500ug	500 ug
EPO mRNA, human	7001-106-1mg	1 mg

 

AMSBIO offer custom mRNA construct services for further information please contact us.

NEW - Reprogramming Qualified Human Fibroblasts 
 

Description	Datasheet	Pack Size	Order
Human Feeder Fibroblasts (NuFF-RQ) Irradiated - iPSC Reprogramming Qualified		4-5 M cells/vial

 

Warren, e. a. (2010). Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Cell Stem Cell, 7, 618-630.

 

Visit our stem cell research tools pages.

AMSBIO’s mission is to be a profitable premier provider of quality life science research reagents and services helping customers develop innovative methods, processes, products and medicines. This is achieved by offering small and medium size manufacturers, academic groups and revenue generating biotechs a unique partnership for the global market and by providing state of the art and cost effective solutions to end users and partners.

Formed in 1987 by Sandy Allan and Alex Sim the company’s first offices were in Madrid, Spain. Over the following three years further offices were opened in Italy, Switzerland and the UK. During the formative years of existence AMS helped launch the successful sales campaigns of Stratagene and Invitrogen providing them with an important foothold from which to develop their global brand expansion.